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Proteintech anti cdk6
Anti Cdk6, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 312 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cdk6/CDK6+Antibody/pmc12973475-77-74-75
Average 96 stars, based on 312 article reviews
anti cdk6 - by Bioz Stars, 2026-10
96/100 stars

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Related Articles

other:

Article Title: HDAC2 promotes malignant progression by the RBM47/NONO axis in medulloblastoma
Article Snippet: Anti-CDK6 , 14,052–1-AP , Proteintech (Wuhan, China).

Blocking Assay:

Article Title: SNX1 inhibits human ovarian cancer progression via regulation of the cell cycle, apoptosis and migration.
Article Snippet: Equal amounts of protein (30 μg) were separated by 10% SDS-PAGE (G2043-50T, Servicebio) and transferred to nitrocellulose membranes (Millipore). .. After blocking with 5% skimmed milk, the membranes were incubated overnight at 4 °C with the following primary antibodies diluted in TBST containing 1% BSA: anti-beta-Actin (20536-1-AP, 1:1000, Proteintech), anti-SNX1 (14914-1-AP, 1:1000, Proteintech), anti-CDK2 (10122-1-AP, 1:1000, Proteintech), anti-CDK6 (14052-1-AP, 1:1000, Proteintech), anti-Cyclin D1 (60186-1-AP, 1:1000, Proteintech), anti-E2F1 (12171-1- AP, 1:1000, Proteintech), anti-GAPDH (60004-1-Ig, 1:5000, Proteintech), followed by 1-hour incubation with corresponding HRP-conjugated secondary antibodies (Goat Anti-Rabbit: HA1001 or Goat Anti-Mouse: HA1006, 1:10000, Huabio) at room temperature. .. Protein bands were visualized using an ECL kit (P0018S, Beyotime) and imaged with a chemiluminescence system (Bio-Rad, U.S.A.).

Article Title: SNX1 inhibits human ovarian cancer progression via regulation of the cell cycle, apoptosis and migration
Article Snippet: Equal amounts of protein (30 μg) were separated by 10% SDS-PAGE (G2043-50T, Servicebio) and transferred to nitrocellulose membranes (Millipore). .. After blocking with 5% skimmed milk, the membranes were incubated overnight at 4 °C with the following primary antibodies diluted in TBST containing 1% BSA: anti-beta-Actin (20536-1-AP, 1:1000, Proteintech), anti-SNX1 (14914-1-AP, 1:1000, Proteintech), anti-CDK2 (10122-1-AP, 1:1000, Proteintech), anti-CDK6 (14052-1-AP, 1:1000, Proteintech), anti-Cyclin D1 (60186-1-AP, 1:1000, Proteintech), anti-E2F1 (12171-1-AP, 1:1000, Proteintech), anti-GAPDH (60004-1-Ig, 1:5000, Proteintech), followed by 1-hour incubation with corresponding HRP-conjugated secondary antibodies (Goat Anti-Rabbit: HA1001 or Goat Anti-Mouse: HA1006, 1:10000, Huabio) at room temperature. .. Protein bands were visualized using an ECL kit (P0018S, Beyotime) and imaged with a chemiluminescence system (Bio-Rad, U.S.A.).

Incubation:

Article Title: SNX1 inhibits human ovarian cancer progression via regulation of the cell cycle, apoptosis and migration.
Article Snippet: Equal amounts of protein (30 μg) were separated by 10% SDS-PAGE (G2043-50T, Servicebio) and transferred to nitrocellulose membranes (Millipore). .. After blocking with 5% skimmed milk, the membranes were incubated overnight at 4 °C with the following primary antibodies diluted in TBST containing 1% BSA: anti-beta-Actin (20536-1-AP, 1:1000, Proteintech), anti-SNX1 (14914-1-AP, 1:1000, Proteintech), anti-CDK2 (10122-1-AP, 1:1000, Proteintech), anti-CDK6 (14052-1-AP, 1:1000, Proteintech), anti-Cyclin D1 (60186-1-AP, 1:1000, Proteintech), anti-E2F1 (12171-1- AP, 1:1000, Proteintech), anti-GAPDH (60004-1-Ig, 1:5000, Proteintech), followed by 1-hour incubation with corresponding HRP-conjugated secondary antibodies (Goat Anti-Rabbit: HA1001 or Goat Anti-Mouse: HA1006, 1:10000, Huabio) at room temperature. .. Protein bands were visualized using an ECL kit (P0018S, Beyotime) and imaged with a chemiluminescence system (Bio-Rad, U.S.A.).

Article Title: SNX1 inhibits human ovarian cancer progression via regulation of the cell cycle, apoptosis and migration
Article Snippet: Equal amounts of protein (30 μg) were separated by 10% SDS-PAGE (G2043-50T, Servicebio) and transferred to nitrocellulose membranes (Millipore). .. After blocking with 5% skimmed milk, the membranes were incubated overnight at 4 °C with the following primary antibodies diluted in TBST containing 1% BSA: anti-beta-Actin (20536-1-AP, 1:1000, Proteintech), anti-SNX1 (14914-1-AP, 1:1000, Proteintech), anti-CDK2 (10122-1-AP, 1:1000, Proteintech), anti-CDK6 (14052-1-AP, 1:1000, Proteintech), anti-Cyclin D1 (60186-1-AP, 1:1000, Proteintech), anti-E2F1 (12171-1-AP, 1:1000, Proteintech), anti-GAPDH (60004-1-Ig, 1:5000, Proteintech), followed by 1-hour incubation with corresponding HRP-conjugated secondary antibodies (Goat Anti-Rabbit: HA1001 or Goat Anti-Mouse: HA1006, 1:10000, Huabio) at room temperature. .. Protein bands were visualized using an ECL kit (P0018S, Beyotime) and imaged with a chemiluminescence system (Bio-Rad, U.S.A.).

Article Title: Glucosamine induces apoptosis of cholangiocarcinoma cells by suppressing high-mannose type N -glycosylation and EGFR/STAT3 signaling
Article Snippet: A total of 20–30 μg protein was separated on 12% sodium dodecyl sulfate-gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (Immobilon-P; Millipore, MA). .. Membranes were blocked with 5% skim milk (w/v) in Tris-buffered saline with 0.1% Tween 20 (TBST), and then incubated with primary antibodies included anti-X-linked inhibitor of apoptosis protein (XIAP) (Santa Cruz Biotechnology, Dallas, TX), anti-cyclin D1 (Cell Signaling, Cambridge, MA), anti-p21 (Cell Signaling), anti-caspase 9 (Cell Signaling), anti-caspase 3 (Cell Signaling), anti-cleaved caspase 3 (Cell Signaling), anti-Poly (ADP-ribose) polymerase (PARP) (Cell signaling), anti-cleaved PARP (cell signaling), anti-cyclin dependent kinase (CDK) 4 (Proteintech, Rosemont, IL), anti-CDK6 (Proteintech), anti-epidermal growth factor receptor (EGFR) (Cell Signaling), anti-signal transducer and activator of transcription 3 (STAT3) (Cell Signaling), anti-pSTAT3 (Y075) (Cell Signaling), and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EMD Millipore, Darmstadt, Germany). ..

Saline:

Article Title: Glucosamine induces apoptosis of cholangiocarcinoma cells by suppressing high-mannose type N -glycosylation and EGFR/STAT3 signaling
Article Snippet: A total of 20–30 μg protein was separated on 12% sodium dodecyl sulfate-gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (Immobilon-P; Millipore, MA). .. Membranes were blocked with 5% skim milk (w/v) in Tris-buffered saline with 0.1% Tween 20 (TBST), and then incubated with primary antibodies included anti-X-linked inhibitor of apoptosis protein (XIAP) (Santa Cruz Biotechnology, Dallas, TX), anti-cyclin D1 (Cell Signaling, Cambridge, MA), anti-p21 (Cell Signaling), anti-caspase 9 (Cell Signaling), anti-caspase 3 (Cell Signaling), anti-cleaved caspase 3 (Cell Signaling), anti-Poly (ADP-ribose) polymerase (PARP) (Cell signaling), anti-cleaved PARP (cell signaling), anti-cyclin dependent kinase (CDK) 4 (Proteintech, Rosemont, IL), anti-CDK6 (Proteintech), anti-epidermal growth factor receptor (EGFR) (Cell Signaling), anti-signal transducer and activator of transcription 3 (STAT3) (Cell Signaling), anti-pSTAT3 (Y075) (Cell Signaling), and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (EMD Millipore, Darmstadt, Germany). ..

Western Blot:

Article Title: A comprehensive analysis of deubiquitinase <scp>USP20</scp> on prognosis and immunity in pan‐cancer
Article Snippet: .. Western blotting was performed as described previously.29 The primary antibodies used for Western blotting included anti- USP20 (17491- 1- AP, Proteintech), anti- ZEB1 (21544- 1- AP, Proteintech), anti- E- cadherin (20874- 1- AP, Proteintech), anti- FLAG (66008- 3- Ig, Proteintech), antiGST (GB12085, Servicebio), anti- c- MYC (67447- 1- Ig, Proteintech), anti- Cyclin D1 (60186- 1- Ig, Proteintech), anti- CDK4 (11026- 1- AP, Proteintech), anti- CDK6 (14052- 1- AP, Proteintech), P21 (Cell Signaling Technology, #2947), and anti- β- actin (GB12001, Servicebio) antibodies. .. The dilution ratio of HRP- linked anti- IgG as a secondary antibody was 1:5000 (Zhongshan Company). nloaded from https://faseb.onlinelibrary.w iley.com /doi/10.1096/fj.202402603R by N guyen T am - T aipei M edical U niversity(T m u) , W iley O nline L ibrary on [12/09/2025].



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